Microscope Control Initialization Succeeded. Dynamic camera registered. Welcome to DigitalMicrograph. 27.09.2006, 13:52:44 Automatic focussing started at: 27.09.2006 14:00:57 Microscope magnification: 150000x Exposure time (sec): 0.050 Binning: 4 Image size (pixels): 256 x 256 Start microscope calibration at 150 kx. Start focus calibration. 16-bit DAC range Max DAC change = ± 32767 Searching for optimal tilt: Induced tilt: 0.29 mrad Image shift: 0.74 pixels Induced beam tilt: 1.0 mrad Tilt (X): Image shift = (0.50,0.07) pixels Tilt (Y): Image shift = (0.26,0.04) pixels Without induced change in focus or astigmatism: Image shift (x-tilt) = 0.502311 pixels Finding proper DAC value for induced focus change. Tilt (X): Image shift = (-0.03,-0.94) pixels Image shift (induced focus change) = 0.942327 pixels Tilt (X): Image shift = (-2.22,-0.68) pixels Image shift (induced focus change) = 2.32546 pixels Tilt (X): Image shift = (-21.92,-10.88) pixels Image shift (induced focus change) = 24.4721 pixels Induced focus change (DAC) = -6582.03 Tilt (Y): Image shift = (-13.04,9.17) pixels Focus calibration = 0.874818 nm/DAC. Focus calibration is complete. Finding proper DAC value for induced astigmatism change. Tilt (X): Image shift = (0.65,-1.03) pixels Image shift (induced astigmatism change) = 1.22015 pixels Tilt (X): Image shift = (2.71,-0.63) pixels Image shift (induced astigmatism change) = 2.78222 pixels Tilt (X): Image shift = (7.18,-0.51) pixels Image shift (induced astigmatism change) = 7.19964 pixels Induced stigmator (x) change (DAC) = -26316.9 Tilt (Y): Image shift = (-1.76,5.43) pixels x-stigmator calibration = 0.0670357 nm/DAC x-stigmator calibration is complete. Tilt (X): Image shift = (1.12,-7.55) pixels Tilt (Y): Image shift = (6.81,2.36) pixels y-stigmator calibration = 0.0776923 nm/DAC Average stigmator strength = 0.072364 nm/DAC y-stigmator calibration is complete. Induced beam tilt: 1.0 mrad Image shift = (-0.06,-1.06) pixels Microscope adjustment: Defocus: -25 Automatic focussing finished at: 27.09.2006 14:01:59